LITE microscopy: Tilted light-sheet excitation of model organisms offers high resolution and low photobleaching.

ABSTRACT: Fluorescence microscopy is a powerful approach for studying subcellular dynamics at high spatiotemporal resolution; however, conventional fluorescence microscopy techniques are light-intensive and introduce unnecessary photodamage. Light-sheet fluorescence microscopy (LSFM) mitigates these problems by selectively illuminating the focal plane of the detection objective by using orthogonal excitation. Orthogonal excitation requires geometries that physically limit the detection objective numerical aperture (NA), thereby limiting both light-gathering efficiency (brightness) and native spatial resolution. We present a novel live-cell LSFM method, lateral interference tilted excitation (LITE), in which a tilted light sheet illuminates the detection objective focal plane without a sterically lim

SUBMITTER: Fadero TC 

PROVIDER: S-EPMC5940309 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature