PI(4,5)P₂ controls plasma membrane PI4P and PS levels via ORP5/8 recruitment to ER-PM contact sites.

ABSTRACT: Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P₂) is a critically important regulatory lipid of the plasma membrane (PM); however, little is known about how cells regulate PM PI(4,5)P₂ levels. Here, we show that the phosphatidylinositol 4-phosphate (PI4P)/phosphatidylserine (PS) transfer activity of the endoplasmic reticulum (ER)-resident ORP5 and ORP8 is regulated by both PM PI4P and PI(4,5)P₂ Dynamic control of ORP5/8 recruitment to the PM occurs through interactions with the N-terminal Pleckstrin homology domains and adjacent basic residues of ORP5/8 with both PI4P and PI(4,5)P₂ Although ORP5 activity requires normal levels of these inositides, ORP8 is called on only when PI(4,5)P₂ levels are increased. Regulation of the ORP5/8 atta

SUBMITTER: Sohn M 

PROVIDER: S-EPMC5940310 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature