Sika deer (Cervus nippon)-specific real-time PCR method to detect fraudulent labelling of meat and meat products.

ABSTRACT: Since game meat is more valuable and expensive than meat from domesticated animal species it is a potential target for adulteration. Analytical methods must allow the identification and quantification of meat species to be applicable for the detection of fraudulent labelling. We developed a real-time PCR assay for the authentication of sika deer (Cervus nippon) and products thereof. The primer/probe system amplifies a 71 bp fragment of the kappa-casein precursor gene. Since the target sequence contained only one sika deer-specific base, we introduced a deliberate base mismatch in the forward primer. The real-time PCR assay did not show cross-reactivity with 19 animal and 49 plant species tested. Low cross-reactivity was observed with red deer, fallow deer, reindeer and moose. However, with

SUBMITTER: Kaltenbrunner M 

PROVIDER: S-EPMC5940659 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature