The influence of different pH on the electrophoretic behaviour of Saccharomyces cerevisiae modified by calcium ions.

ABSTRACT: The effect of a different pH on Saccharomyces cerevisiae cells modified with calcium ions was investigated by the capillary zone electrophoresis technique. For the identification of the wild strain of S. cerevisiae, the ribosomal nucleic acid sequencing and internal transcribed spacer sequencing as well as spectrometric approach were applied. The potentiometric titration and Fourier transform infrared spectroscopy have shown the occurrence of active functional groups such as carboxyl, amine/hydroxyl, phosphate/hydrogen phosphate groups on the surface of native yeast cells. Moreover, the spectroscopy study in a medium infrared range was carried out to identify the functional groups of yeast cells that participate in calcium ions binding interaction. Furthermore, the microscopic and spectrom

SUBMITTER: Rogowska A 

PROVIDER: S-EPMC5940755 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature