Consensus Bayesian assessment of protein molecular mass from solution X-ray scattering data.

ABSTRACT: Molecular mass (MM) is one of the key structural parameters obtained by small-angle X-ray scattering (SAXS) of proteins in solution and is used to assess the sample quality, oligomeric composition and to guide subsequent structural modelling. Concentration-dependent assessment of MM relies on a number of extra quantities (partial specific volume, calibrated intensity, accurate solute concentration) and often yields limited accuracy. Concentration-independent methods forgo these requirements being based on the relationship between structural parameters, scattering invariants and particle volume obtained directly from the data. Using a comparative analysis on 165,982 unique scattering profiles calculated from high-resolution protein structures, the performance of multiple concentration-indep

SUBMITTER: Hajizadeh NR 

PROVIDER: S-EPMC5940760 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature