Functional insights into the interplay between DNA interaction and metal coordination in ferric uptake regulators.

ABSTRACT: Ferric uptake regulators (Fur) are a family of transcription factors coupling gene regulatory events to metal concentration. Recent evidence has expanded the mechanistic repertoires employed by Fur to activate or repress gene expression in the presence or absence of regulatory metals. However, the mechanistic basis underlying this extended repertoire has remained largely unexplored. In this study, we used an extensive set of mutations to demonstrate that Campylobacter jejuni Fur (CjFur) employs the same surface to positively and negatively control gene expression regardless of the presence or absence of metals. Moreover, the crystal structure determination of a CjFur devoid of any regulatory metals shows that subtle reorientation of the transcription factor DNA binding domain negatively im

SUBMITTER: Sarvan S 

PROVIDER: S-EPMC5940780 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature