NOTCH signaling specifies arterial-type definitive hemogenic endothelium from human pluripotent stem cells.

ABSTRACT: NOTCH signaling is required for the arterial specification and formation of hematopoietic stem cells (HSCs) and lympho-myeloid progenitors in the embryonic aorta-gonad-mesonephros region and extraembryonic vasculature from a distinct lineage of vascular endothelial cells with hemogenic potential. However, the role of NOTCH signaling in hemogenic endothelium (HE) specification from human pluripotent stem cell (hPSC) has not been studied. Here, using a chemically defined hPSC differentiation system combined with the use of DLL1-Fc and DAPT to manipulate NOTCH, we discover that NOTCH activation in hPSC-derived immature HE progenitors leads to formation of CD144⁺CD43^-CD73^-DLL4⁺Runx1 + 23-GFP⁺ arterial-type HE, which requires NOTCH signalin

SUBMITTER: Uenishi GI 

PROVIDER: S-EPMC5940870 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature