Genetic Variability of Antigen B8/1 among Echinococcus granulosus Isolates from Human, Cattle, and Sheep in Fars Province, Southern Iran.

ABSTRACT:

Background

Cystic echinococcosis (CE), known as hydatid cyst, is a zoonotic parasitic infection caused by the larval stage of Echinococcus granulosus (E. granulosus). Antigen B, the major component of hydatid cyst fluid, is encoded by members of a multigene family. The present study aimed to evaluate the genetic diversity of the gene encoding antigen B8/1 (EgAgB8/1) among the main intermediate hosts of E. granulosus.

Methods

Twenty-eight hydatid cyst isolates (10 sheep, 9 human, and 9 cattle) were collected in Fars province, Iran. DNA was extracted from each cyst and PCR, followed by DNA sequencing was used to identify potential EgAgB8/1 sequence variation and polymorphism. A phylogenetic tree was constructed using MEGA 7.0 software and the maximu

SUBMITTER: Fatemi Esfedan A 

PROVIDER: S-EPMC5941130 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature