Exploration of genetic basis underlying individual differences in radiosensitivity within human populations using genome editing technology.

ABSTRACT: DNA double-strand breaks (DSBs) induced by ionizing radiation (IR) are the initial and critical step in major alteration of genetic information and cell death. To prevent deleterious effects, DNA repair systems recognize and re-join DNA DSBs in human cells. It has been suggested that there are individual differences in radiosensitivity within human populations, and that variations in DNA repair genes might contribute to this heterogeneity. Because confounding factors, including age, gender, smoking, and diverse genetic backgrounds within human populations, also influence the cellular radiosensitivity, to accurately measure the effect of candidate genetic variations on radiosensitivity, it is necessary to use human cultured cells with a uniform genetic background. However, a reverse genetic

SUBMITTER: Miyamoto T 

PROVIDER: S-EPMC5941146 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature