A genome-wide survey of mutations in the Jurkat cell line.

ABSTRACT:

Background

The Jurkat cell line has an extensive history as a model of T cell signaling. But at the turn of the 21st century, some expression irregularities were observed, raising doubts about how closely the cell line paralleled normal human T cells. While numerous expression deficiencies have been described in Jurkat, genetic explanations have only been provided for a handful of defects.

Results

Here, we report a comprehensive catolog of genomic variation in the Jurkat cell line based on whole-genome sequencing. With this list of all detectable, non-reference sequences, we prioritize potentially damaging mutations by mining public databases for functional effects. We confirm documented mutations in Jurkat and propose links from detrimental gene variants to observed express

SUBMITTER: Gioia L 

PROVIDER: S-EPMC5941560 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature