Circular RNA expression and regulatory network prediction in posterior cingulate astrocytes in elderly subjects.

ABSTRACT:

Background

Circular RNAs (circRNAs) are a novel class of endogenous, non-coding RNAs that form covalently closed continuous loops and that are both highly conserved and abundant in the mammalian brain. A role for circRNAs in sponging microRNAs (miRNAs) has been proposed, but the circRNA-miRNA-mRNA interaction networks in human brain cells have not been defined. Therefore, we identified circRNAs in RNA sequencing data previously generated from astrocytes microdissected from the posterior cingulate (PC) of Alzheimer's disease (AD) patients (N = 10) and healthy elderly controls (N = 10) using four circRNA prediction algorithms - CIRI, CIRCexplorer, find_circ and KNIFE.

Results

Overall, utilizing these four tools, we identified a union of 4438 unique circRNAs across all samples,

SUBMITTER: Sekar S 

PROVIDER: S-EPMC5941680 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature