Cryopreservation without vitrification suitable for large scale cryopreservation of orchid seeds.

ABSTRACT:

Background

Orchids are under threat from human activities and climate change, with populations limited to small geographic hotspots. This makes them ideal candidates for ex situ conservation. Orchid seeds are desiccation tolerant, but often have poor longevity in seed banks and cryopreservation of orchid protocorms is complex and expensive. Therefore, simple methods for large-scale storage programs are essential to store orchid seeds of different life forms. Seeds of five species representing epiphytic, lithophytic and terrestrial orchids from the Central Highlands of Madagascar were studied to find a simple and effective system of cryopreservation. The use of a vitrification solution prior to cryopreservation to improve survival was investigated, as well as the use of symbiotic an

SUBMITTER: Schofield E 

PROVIDER: S-EPMC5943204 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature