Enterovirus 71 infection of human airway organoids reveals VP1-145 as a viral infectivity determinant.

ABSTRACT: Human enteroviruses frequently cause severe diseases in children. Human enteroviruses are transmitted via the fecal-oral route and respiratory droplets, and primary replication occurs in the gastro-intestinal and respiratory tracts; however, how enteroviruses infect these sites is largely unknown. Human intestinal organoids have recently proven to be valuable tools for studying enterovirus-host interactions in the intestinal tract. In this study, we demonstrated the susceptibility of a newly developed human airway organoid model for enterovirus 71 (EV71) infection. We showed for the first time in a human physiological model that EV71 replication kinetics are strain-dependent. A glutamine at position 145 of the VP1 capsid protein was identified as a key determinant of infectivity, and resid

SUBMITTER: van der Sanden SMG 

PROVIDER: S-EPMC5943241 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature