Relevance of arginine residues in Cu(II)-induced DNA breakage and Proteinase K resistance of H1 histones.

ABSTRACT: This work analyzes the involvement of arginines in copper/H₂O₂-induced DNA breakage. Copper is a highly redox active metal which has been demonstrated to form compounds with arginines. For this aim we used mixtures of pGEM3 DNA plasmid and two types of H1 histones which differ only in their arginine content. The sperm H1 histone from the annelid worm Chaetopterus variopedatus (arginine content 12.6 mol% K/R ratio 2) and the somatic H1 histone from calf thymus (arginine content 1.8 mol% and K/R ratio 15). Copper/H₂O₂-induced DNA breakage was observed only in presence of sperm H1 histones, but it was more relevant for the native molecule than for the deguanidinated derivative (K/R ratio 14), in which 80% of arginine residues were converted to ornit

SUBMITTER: Piscopo M 

PROVIDER: S-EPMC5943286 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature