Analysis of early changes in DNA methylation in synovial fibroblasts of RA patients before diagnosis.

ABSTRACT: DNA methylation is an important epigenetic modification that is known to be altered in rheumatoid arthritis synovial fibroblasts (RASF). Here, we compared the status of promoter DNA methylation of SF from patients with very early RA with SF from patients with resolving arthritis, fully established RA and from non-arthritic patients. DNA was hybridized to Infinium Human methylation 450k and 850k arrays and differential methylated genes and pathways were identified. We could identify a significant number of CpG sites that differed between the SF of different disease stages, showing that epigenetic changes in SF occur early in RA development. Principal component analysis confirmed that the different groups of SF were separated according to their DNA methylation state. Furthermore, pathway ana

SUBMITTER: Karouzakis E 

PROVIDER: S-EPMC5943364 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature