Calretinin Participates in Regulating Steroidogenesis by PLC-Ca²⁺-PKC Pathway in Leydig Cells.

ABSTRACT: Calretinin, a Ca²⁺-binding protein, participates in many cellular events. Our previous studies found the high expression of calretinin in testicular Leydig cells. In this study, (MLTC-1 cells were infected with LV-calb2, R2C cells with LV-siRNA-calb2. The primary mouse Leydig cells were also used to confirm those data from cell lines. Testosterone level was significantly higher in the MLTC-1 cells with over-expressed calretinin than in the control, while progesterone was lower in the R2C cells in which down-regulated calretinin. The expressions of StAR changed in synchrony with hormones. Cytoplasmic Ca²⁺ level was significantly increased when calretinin was over-expressed. When MLTC-1 cells were infected with LV-calb2 and then stimulated using Clopiazonic, a Ca²⁺

SUBMITTER: Xu W 

PROVIDER: S-EPMC5943404 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature