Methylglyoxal Disrupts Paranodal Axoglial Junctions via Calpain Activation.

ABSTRACT: Nodes of Ranvier and associated paranodal and juxtaparanodal domains along myelinated axons are essential for normal function of the peripheral and central nervous systems. Disruption of these domains as well as increases in the reactive carbonyl species methylglyoxal are implicated as a pathophysiology common to a wide variety of neurological diseases. Here, using an ex vivo nerve exposure model, we show that increasing methylglyoxal produces paranodal disruption, evidenced by disorganized immunostaining of axoglial cell-adhesion proteins, in both sciatic and optic nerves from wild-type mice. Consistent with previous studies showing that increase of methylglyoxal can alter intracellular calcium homeostasis, we found upregulated activity of the calcium-activated protease calpain in sciatic

SUBMITTER: Griggs RB 

PROVIDER: S-EPMC5944142 | biostudies-literature | 2018 Jan-Dec

REPOSITORIES: biostudies-literature