Cloning and characterization of enoate reductase with high β-ionone to dihydro-β-ionone bioconversion productivity.

ABSTRACT:

Background

Dihydro-β-ionone is a principal aroma compound and has received considerable attention by flavor and fragrance industry. The traditional method of preparing dihydro-β-ionone has many drawbacks, which has restricted its industrial application. Therefore, it is necessary to find a biotechnological method to produce dihydro-β-ionone.

Results

In this study, the enoate reductase with high conversion efficiency of β-ionone to dihydro-β-ionone, DBR1, was obtained by screening four genetically engineered bacteria. The product, dihydro-β-ionone, was analyzed by GC and GC-MS. The highest dihydro-β-ionone production with 308.3 mg/L was detected in the recombinant strain expressing DBR1 which was later on expressed and purified. Its optimal temperature and pH were 45 °C and 6

SUBMITTER: Zhang X 

PROVIDER: S-EPMC5944158 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature