Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals.

ABSTRACT: Neurons are highly polarized cells that require continuous turnover of membrane proteins at axon terminals to develop, function, and survive. Yet, it is still unclear whether membrane protein degradation requires transport back to the cell body or whether degradation also occurs locally at the axon terminal, where live observation of sorting and degradation has remained a challenge. Here, we report direct observation of two cargo-specific membrane protein degradation mechanisms at axon terminals based on a live-imaging approach in intact Drosophila brains. We show that different acidification-sensing cargo probes are sorted into distinct classes of degradative "hub" compartments for synaptic vesicle proteins and plasma membrane proteins at axon terminals. Sorting and degradation of the two

SUBMITTER: Jin EJ 

PROVIDER: S-EPMC5944365 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature