Metabolic alterations in urine extracellular vesicles are associated to prostate cancer pathogenesis and progression.

ABSTRACT: Urine contains extracellular vesicles (EVs) that concentrate molecules and protect them from degradation. Thus, isolation and characterisation of urinary EVs could increase the efficiency of biomarker discovery. We have previously identified proteins and RNAs with differential abundance in urinary EVs from prostate cancer (PCa) patients compared to benign prostate hyperplasia (BPH). Here, we focused on the analysis of the metabolites contained in urinary EVs collected from patients with PCa and BPH. Targeted metabolomics analysis of EVs was performed by ultra-high-performance liquid chromatography-mass spectrometry. The correlation between metabolites and clinical parameters was studied, and metabolites with differential abundance in PCa urinary EVs were detected and mapped into cellular p

SUBMITTER: Clos-Garcia M 

PROVIDER: S-EPMC5944373 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature