Kinetic studies on strand displacement in de novo designed parallel heterodimeric coiled coils.

ABSTRACT: Among the protein folding motifs, which are accessible by de novo design, the parallel heterodimeric coiled coil is most frequently used in bioinspired applications and chemical biology in general. This is due to the straightforward sequence-to-structure relationships, which it has in common with all coiled-coil motifs, and the heterospecificity, which allows control of association. Whereas much focus was laid on designing orthogonal coiled coils, systematic studies on controlling association, for instance by strand displacement, are rare. As a contribution to the design of dynamic coiled-coil-based systems, we studied the strand-displacement mechanism in obligate heterodimeric coiled coils to investigate the suitability of the dissociation constants (K_D) as parame

SUBMITTER: Groth MC 

PROVIDER: S-EPMC5944379 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature