Variation in DNA-Damage Responses to an Inhalational Carcinogen (1,3-Butadiene) in Relation to Strain-Specific Differences in Chromatin Accessibility and Gene Transcription Profiles in C57BL/6J and CAST/EiJ Mice.

ABSTRACT:

Background

The damaging effects of exposure to environmental toxicants differentially affect genetically distinct individuals, but the mechanisms contributing to these differences are poorly understood. Genetic variation affects the establishment of the gene regulatory landscape and thus gene expression, and we hypothesized that this contributes to the observed heterogeneity in individual responses to exogenous cellular insults.

Objectives

We performed an in vivo study of how genetic variation and chromatin organization may dictate susceptibility to DNA damage, and influence the cellular response to such damage, caused by an environmental toxicant.

Materials and methods

We measured DNA damage, messenger RNA (mRNA) and microRNA (miRNA) expression, and genome-wid

SUBMITTER: Chappell GA 

PROVIDER: S-EPMC5944832 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature