Evaluation of a novel non-invasive preimplantation genetic screening approach.

ABSTRACT:

Objective

To assess whether embryonic DNA isolated from blastocyst culture conditioned medium (BCCM) combined with blastocoel fluid (BF) could be used for blastocyst stage non-invasive preimplantation genetic testing for chromosomal aneuploidy (non-invasive preimplantation genetic screening, NIPGS).

Patients

47 embryos from 35 patients undergoing IVF.

Interventions

DNA analysis of combined BCCM plus BF in comparison with trophectoderm (TE) biopsy and/or whole blastocyst (WB)using next generation sequencing (NGS).

Results

Embryonic DNA was successfully amplified in 47/47 NIPGS samples (28 frozen-thawed and 19 fresh culture samples) ranging from 6.3 to 44.0 ng/μl. For frozen-thawed embryos, the concordance rate for whole chromosome copy number per sample was equi

SUBMITTER: Kuznyetsov V 

PROVIDER: S-EPMC5944986 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature