NanoSIMS Analysis of Intravascular Lipolysis and Lipid Movement across Capillaries and into Cardiomyocytes.

ABSTRACT: The processing of triglyceride-rich lipoproteins (TRLs) in capillaries provides lipids for vital tissues, but our understanding of TRL metabolism is limited, in part because TRL processing and lipid movement have never been visualized. To investigate the movement of TRL-derived lipids in the heart, mice were given an injection of [²H]triglyceride-enriched TRLs, and the movement of ²H-labeled lipids across capillaries and into cardiomyocytes was examined by NanoSIMS. TRL processing and lipid movement in tissues were extremely rapid. Within 30 s, TRL-derived lipids appeared in the subendothelial spaces and in the lipid droplets and mitochondria of cardiomyocytes. Enrichment of ²H in capillary endothelial cells was not greater than in cardiomyocytes, implying

SUBMITTER: He C 

PROVIDER: S-EPMC5945212 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature