Presenilin PS1∆E9 disrupts mobility of secretory organelles in rat astrocytes.

ABSTRACT:

Aim

Alzheimer's disease (AD) is largely considered a neuron-derived insult, but also involves failure of astroglia. A recent study indicated that mutated presenilin 1 (PS1M146V), a putative endoplasmic reticulum (ER) Ca²⁺ channel with decreased Ca²⁺ conductance, impairs the traffic of astroglial peptidergic vesicles. Whether other pathogenically relevant PS1 mutants, such as PS1ΔE9, which code for ER channel with putative increased Ca²⁺ conductance, similarly affect vesicle traffic, is unknown.

Methods

Here, we cotransfected rat astrocytes with plasmids encoding mutant PS1ΔE9 and atrial natriuretic peptide or vesicular glutamate transporter 1 tagged with fluorescent proteins (pANP.emd or pVGLUT1-EGFP respectively), to microscopically examine

SUBMITTER: Stenovec M 

PROVIDER: S-EPMC5945314 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature