Experimental asthma persists in IL-33 receptor knockout mice because of the emergence of thymic stromal lymphopoietin-driven IL-9⁺ and IL-13⁺ type 2 innate lymphoid cell subpopulations.

ABSTRACT:

Background

IL-33 plays an important role in the development of experimental asthma.

Objective

We sought to study the role of the IL-33 receptor suppressor of tumorigenicity 2 (ST2) in the persistence of asthma in a mouse model.

Methods

We studied allergen-induced experimental asthma in ST2 knockout (KO) and wild-type control mice. We measured airway hyperresponsiveness by using flexiVent; inflammatory indices by using ELISA, histology, and real-time PCR; and type 2 innate lymphoid cells (ILC2s) in lung single-cell preparations by using flow cytometry.

Results

Airway hyperresponsiveness was increased in allergen-treated ST2 KO mice and comparable with that in allergen-treated wild-type control mice. Peribronchial and perivascular inflammation and mucus productio

SUBMITTER: Verma M 

PROVIDER: S-EPMC5945345 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature