Identification of copy number alterations in colon cancer from analysis of amplicon-based next generation sequencing data.

ABSTRACT: The objective of this study was to determine the feasibility to detect copy number alterations in colon cancer samples using Next Generation Sequencing data and to elucidate the association between copy number alterations in specific genes and the development of cancer in different colon segments. We report the successful detection of somatic changes in gene copy number in 37 colon cancer patients by analysis of sequencing data through Amplicon CNA Algorithm. Overall, we have found a total of 748 significant copy number alterations in 230 significant genes, of which 143 showed CN losses and 87 showed CN gains. Validation of results was performed on 20 representative genes by quantitative qPCR and/or immunostaining. By this analysis, we have identified 4 genes that were subjected to copy nu

SUBMITTER: Oliveira DM 

PROVIDER: S-EPMC5945505 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature