Electrophysiological properties of anion exchangers in the luminal membrane of guinea pig pancreatic duct cells.

ABSTRACT: The pancreatic duct epithelium secretes the HCO3--rich pancreatic juice. The HCO3- transport across the luminal membrane has been proposed to be mediated by SLC26A Cl--HCO3- exchangers. To examine the electrophysiological properties of Cl--HCO3- exchangers, we directly measured HCO3- conductance in the luminal membrane of the interlobular pancreatic duct cells from guinea pigs using an inside-out patch-clamp technique. Intracellular HCO3- increased the HCO3- conductance with a half-maximal effective concentration value of approximately 30 mM. The selectivity sequence based on permeability ratios was SCN- (1.4) > Cl- (1.2) = gluconate (1.1) = I- (1.1) = HCO3- (1.0) > methanesulfonate (0.6). The sequence of the relative conductance was HCO3- (1.0) > SCN- (0.7) = I- (0.7) > Cl- (0.5) = glucon

SUBMITTER: Andharia N 

PROVIDER: S-EPMC5945753 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature