A simple and fast method for fixation of cultured cell lines that preserves cellular structures containing gamma-tubulin.

ABSTRACT: When using fluorescence microscope techniques to study cells, it is essential that the cell structure and contents are preserved after preparation of the samples, and that the preparation method employed does not create artefacts that can be perceived as cellular structure/components. γ-Tubulin forms filaments that in some cases are immunostained with an anti-γ-tubulin antibody, but this immunostaining is not reproducible [[1], [2]]. In addition, the C terminal region of γ-tubulin (green fluorescence protein tagged [GFP]-γ-tubulin334--449) forms cytosolic GFP-labeled structures, which can easily be imaged in live cells but are not preserved in fixed cells [[1], [3]]. The purpose of this study was to identify a fixation technique that preserves cellular constituents containing γ-tubulin. •T

SUBMITTER: Alvarado-Kristensson M 

PROVIDER: S-EPMC5945921 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature