Reactive glia promote development of CD103+ CD69+ CD8+ T-cells through programmed cell death-ligand 1 (PD-L1).

ABSTRACT: INTRODUCTION:Previous work from our laboratory has demonstrated in vivo persistence of CD103+ CD69+ brain resident memory CD8+ T-cells (bTRM ) following viral infection, and that the PD-1: PD-L1 pathway promotes development of these TRM cells within the brain. Although glial cells express low basal levels of PD-L1, its expression is upregulated upon IFN-γ-treatment, and they have been shown to modulate antiviral T-cell effector responses through the PD-1: PD-L1 pathway. METHODS:We performed flow cytometric analysis of cells from co-cultures of mixed glia and CD8+ T-cells obtained from wild type mice to investigate the role of glial cells in the development of bTRM . RESULTS:In this study, we show that interactions between reactive glia and anti-CD3 Ab-stimulated CD8+ T-cells promote develo

SUBMITTER: Prasad S 

PROVIDER: S-EPMC5946148 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature