Preclinical safety and immunological efficacy of Alternaria alternata polymerized extracts.

ABSTRACT:

Introduction

Alternaria alternata is a widespread fungi whose allergy is a risk factor for asthma development. The use of a polymerized allergen extract (allergoid) may be safer than native extract based treatments while maintaining efficacy. The objective of this study was to characterize biochemically and immunochemically a new Alternaria alternata allergoid.

Methods

Characterization of native and allergoid extracts was performed by determination of protein content, protein and allergenic profile, biological potency, identification of Alternaria allergens, and Alt a 1 quantification. Safety was evaluated in toxicological assays (Ames test, limit test, and fish embryo acute toxicity test in zebrafish, and maximum tolerated dose and Dose-range finding study in rats). Efficac

SUBMITTER: Morales M 

PROVIDER: S-EPMC5946150 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature