Development of gold nanoparticles biosensor for ultrasensitive diagnosis of foot and mouth disease virus.

ABSTRACT:

Background

Nano-PCR is a recent tool that is used in viral diseases diagnosis. The technique depends on the fundamental effects of gold nanoparticles (AuNPs) and is considered a very effective and sensitive tool in the diagnosis of different diseases including viral diseases. Although several techniques are currently available to diagnose foot and mouth disease virus (FMDV), a highly sensitive, highly specific technique is needed for specific diagnosis of the disease. In the present work, a novel AuNPs biosensor has been designed using thiol-linked oligonucleotides that recognize the conserved 3D gene of FMDV.

Results

The AuNPs-FMDV biosensor specifically recognizes RNA standards of FMDV, but not that of swine vesicular disease virus (SVDV) isolates. The analytical sensitivi

SUBMITTER: Hamdy ME 

PROVIDER: S-EPMC5946443 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature