Epigenomic Alterations and Gene Expression Profiles in Human Respiratory Epithelial Cells Mediated by Hookah and Cigarette Smoke

ABSTRACT: Rationale: The effects of hookah smoke on respiratory epithelia have not been well characterized. Objectives: To characterize and compare the effects of hookah tobacco smoke and conventional cigarette smoke on the epigenome and transcriptome of human respiratory epithelia. Methods: Normal human small airway epithelial cells and cyclin-dependent kinase 4/human telomerase reverse transcriptase–immortalized human bronchial epithelial cells were cultured for 5 days in normal media in the presence or absence of water pipe condensates or cigarette smoke condensates under relevant exposure conditions. CyQUANT assay (Thermo Fisher Scientific), RNA sequencing, quantitative reverse transcriptase–polymerase chain reaction, and Western blotting techniques were used to examine/compare the effects of ho

SUBMITTER: Xiong Y 

PROVIDER: S-EPMC5946508 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature