Fibrocytes are increased in lung and peripheral blood of patients with idiopathic pulmonary fibrosis.

ABSTRACT:

Background

Fibrocytes are implicated in Idiopathic Pulmonary Fibrosis (IPF) pathogenesis and increased proportions in the circulation are associated with poor prognosis. Upon tissue injury, fibrocytes migrate to the affected organ. In IPF patients, circulating fibrocytes are increased especially during exacerbations, however fibrocytes in the lungs have not been examined. Therefore, we sought to evaluate if fibrocytes can be detected in IPF lungs and we compare percentages and phenotypic characteristics of lung fibrocytes with circulating fibrocytes in IPF.

Methods

First we optimized flow cytometric detection circulating fibrocytes using a unique combination of intra- and extra-cellular markers to establish a solid gating strategy. Next we analyzed lung fibrocytes in single

SUBMITTER: Heukels P 

PROVIDER: S-EPMC5946532 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature