CD32-Expressing CD4 T Cells Are Phenotypically Diverse and Can Contain Proviral HIV DNA.

ABSTRACT: Efforts to both characterize and eradicate the HIV reservoir have been limited by the rarity of latently infected cells and the absence of a specific denoting biomarker. CD32a (FcγRIIa) has been proposed to be a marker for an enriched CD4 T cell HIV reservoir, but this finding remains controversial. Here, we explore the expression of CD32 on CD3⁺CD4⁺ cells in participants from two primary HIV infection studies and identify at least three distinct phenotypes (CD32^low, CD32⁺CD14⁺, and CD32^high). Of note, CD4 negative enrichment kits remove the majority of CD4⁺CD32⁺ T cells, potentially skewing subsequent analyses if used. CD32^high CD4 T cells had higher levels of HLA-DR and HIV co-receptor expr

SUBMITTER: Martin GE 

PROVIDER: S-EPMC5946760 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature