Scots pine aminopropyltransferases shed new light on evolution of the polyamine biosynthesis pathway in seed plants.

ABSTRACT:

Background and aims

Polyamines are small metabolites present in all living cells and play fundamental roles in numerous physiological events in plants. The aminopropyltransferases (APTs), spermidine synthase (SPDS), spermine synthase (SPMS) and thermospermine synthase (ACL5), are essential enzymes in the polyamine biosynthesis pathway. In angiosperms, SPMS has evolved from SPDS via gene duplication, whereas in gymnosperms APTs are mostly unexplored and no SPMS gene has been reported. The present study aimed to investigate the functional properties of the SPDS and ACL5 proteins of Scots pine (Pinus sylvestris L.) in order to elucidate the role and evolution of APTs in higher plants.

Methods

Germinating Scots pine seeds and seedlings were analysed for polyamines by high-perfor

SUBMITTER: Vuosku J 

PROVIDER: S-EPMC5946884 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature