A Blood RNA Signature Detecting Severe Disease in Young Dengue Patients at Hospital Arrival.

ABSTRACT:

Background

Early detection of severe dengue can improve patient care and survival. To date, no reliable single-gene biomarker exists. We hypothesized that robust multigene signatures exist.

Methods

We performed a prospective study on Cambodian dengue patients aged 4 to 22 years. Peripheral blood mononuclear cells (PBMCs) were obtained at hospital admission. We analyzed 42 transcriptomic profiles of patients with secondary dengue infected with dengue serotype 1. Our novel signature discovery approach controls the number of included genes and captures nonlinear relationships between transcript concentrations and severity. We evaluated the signature on secondary cases infected with different serotypes using 2 datasets: 22 PBMC samples from additional patients in our cohort and

SUBMITTER: Nikolayeva I 

PROVIDER: S-EPMC5946912 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature