Detection, characterization, and enrollment of donors of Ebola convalescent plasma in Sierra Leone.

ABSTRACT:

Background

Passive therapy with convalescent plasma provides an early opportunity to intervene in Ebola virus disease (EVD). Methods for field screening and selection of potential donors and quantifying plasma antibody are needed.

Study design and methods

Recombinant Ebola virus glycoprotein (EBOV GP) was formatted into immunoglobulin G-capture, competitive, and double-antigen bridging enzyme immunoassays (EIAs). EVD survivors in Freetown, Sierra Leone, were recruited as potential plasma donors and assessed locally using sera alone and/or paired sera and oral fluids (ORFs). Uninfected controls comprised unexposed Gambians and communities in Western Area, Sierra Leone. Antibody neutralization in selected sera was measured retrospectively in a pseudotype virus assay.

Result

SUBMITTER: Tedder RS 

PROVIDER: S-EPMC5947131 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature