A guide to analysis and reconstruction of serial block face scanning electron microscopy data.

ABSTRACT: Serial block face scanning electron microscopy (SBF-SEM) is a relatively new technique that allows the acquisition of serially sectioned, imaged and digitally aligned ultrastructural data. There is a wealth of information that can be obtained from the resulting image stacks but this presents a new challenge for researchers - how to computationally analyse and make best use of the large datasets produced. One approach is to reconstruct structures and features of interest in 3D. However, the software programmes can appear overwhelming, time-consuming and not intuitive for those new to image analysis. There are a limited number of published articles that provide sufficient detail on how to do this type of reconstruction. Therefore, the aim of this paper is to provide a detailed step-by-step p

SUBMITTER: Cocks E 

PROVIDER: S-EPMC5947172 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature