Low-copy nuclear markers in Isoetes (Isoetaceae) identified with transcriptomes.

ABSTRACT:

Premise of the study

Few genetic markers provide phylogenetic information in closely related species of Isoëtes (Isoëtaceae). We describe the development of primers for several putative low-copy nuclear markers to resolve the phylogeny of Isoëtes, particularly in the southeastern United States.

Methods and results

We identified regions of interest in Isoëtes transcriptomes based on low-copy genes in other plants. Primers were designed for these regions and tested with 16 taxa of Isoëtes and one species of Lycopodium. Parts of the pgiC, gapC, and IBR3 gene regions show phylogenetic signal within the North American and Mediterranean clades of Isoëtes.

Conclusions

Transcriptome data prove useful for identific

SUBMITTER: Schafran PW 

PROVIDER: S-EPMC5947607 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature