Generation of Dynamic Combinatorial Libraries Using Hydrazone-Functionalized Surface Mimetics.

ABSTRACT: Dynamic combinatorial chemistry (DCC) represents an approach, whereby traditional supramolecular scaffolds used for protein surface recognition might be exploited to achieve selective high affinity target recognition. Synthesis, in situ screening and amplification under selection pressure allows the generation of ligands, which bear different moieties capable of making multivalent non-covalent interactions with target proteins. Generic tetracarboxyphenyl porphyrin scaffolds bearing four hydrazide moieties have been used to form dynamic combinatorial libraries (DCLs) using aniline-catalyzed reversible hydrazone exchange reactions, in 10 % DMSO, 5 mm NH₄OAc, at pH 6.75. High resolution mass spectrometry (HRMS) was used to monitor library composition and establish conditions under

SUBMITTER: Hewitt SH 

PROVIDER: S-EPMC5947633 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature