Endoglin in human liver disease and murine models of liver fibrosis-A protective factor against liver fibrosis.

ABSTRACT:

Background & aims

Liver fibrosis is the outcome of chronic liver injury. Transforming growth factor-β (TGF-β) is a major profibrogenic cytokine modulating hepatic stellate cell (HSC) activation and extracellular matrix homeostasis. This study analyses the effect of Endoglin (Eng), a TGF-β type III auxiliary receptor, on fibrogenesis in two models of liver injury by HSC-specific endoglin deletion.

Methods

Eng expression was measured in human and murine samples of liver injury. After generating GFAP^Cre(+) Eng^ΔHSC mice, the impact of Endoglin deletion on chronic liver fibrosis was analysed. For in vitro analysis, Eng^flox/flox HSCs were infected with Cre-expressing virus to deplete Endoglin and fibrogenic responses were analysed.

Re

SUBMITTER: Alsamman M 

PROVIDER: S-EPMC5947658 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature