Three-dimensional ultrastructural imaging reveals the nanoscale architecture of mammalian cells.

ABSTRACT: Knowledge of the interactions between nanomaterials and large-size mammalian cells, including cellular uptake, intracellular localization and translocation, has greatly advanced nanomedicine and nanotoxicology. Imaging techniques that can locate nanomaterials within the structures of intact large-size cells at nanoscale resolution play crucial roles in acquiring this knowledge. Here, the quantitative imaging of intracellular nanomaterials in three dimensions was performed by combining dual-energy contrast X-ray microscopy and an iterative tomographic algorithm termed equally sloped tomography (EST). Macrophages with a size of ∼20 µm that had been exposed to the potential antitumour agent [Gd@C₈₂(OH)₂₂] _n were investigated. Large numbers of nanopartic

SUBMITTER: Yao S 

PROVIDER: S-EPMC5947718 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature