Pilot study of placental tissue collection, processing, and measurement procedures for large scale assessment of placental inflammation.

ABSTRACT:

Background

Placental dysfunction is related to many pregnancy complications, but collecting placental specimens for investigation in large scale epidemiologic studies is often infeasible. Standard procedures involving immediate collection after birth and snap freezing are often cost prohibitive. We aimed to collect pilot data regarding the feasibility and precision of a simpler approach, the collection of tissue samples following 24 hours of refrigeration of whole placentae at 4°C, as compared to the "gold standard" of snap freezing excised tissue within 40 minutes of delivery for the assessment of inflammatory cytokines.

Methods

Placentae were collected from 12 women after delivering live-born singleton babies via uncomplicated vaginal delivery. Two placentae were utilized

SUBMITTER: Sjaarda LA 

PROVIDER: S-EPMC5947903 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature