Transcript-indexed ATAC-seq for precision immune profiling.

ABSTRACT: T cells create vast amounts of diversity in the genes that encode their T cell receptors (TCRs), which enables individual clones to recognize specific peptide-major histocompatibility complex (MHC) ligands. Here we combined sequencing of the TCR-encoding genes with assay for transposase-accessible chromatin with sequencing (ATAC-seq) analysis at the single-cell level to provide information on the TCR specificity and epigenomic state of individual T cells. By using this approach, termed transcript-indexed ATAC-seq (T-ATAC-seq), we identified epigenomic signatures in immortalized leukemic T cells, primary human T cells from healthy volunteers and primary leukemic T cells from patient samples. In peripheral blood CD4⁺ T cells from healthy individuals, we identified cis and trans re

SUBMITTER: Satpathy AT 

PROVIDER: S-EPMC5948148 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature