Cre Recombinase Mediates the Removal of Bacterial Backbone to Efficiently Generate rSV40.

ABSTRACT: Gene therapy has been shown to be a feasible approach to treat inherited disorders in vivo. Among the currently used viral vector systems, adeno-associated virus (AAV) vectors are the most advanced and have been applied in patients successfully. An important drawback of non-integrating AAV vectors is their loss of expression upon cell division, while repeating systemic administration lacks efficacy due to the induction of neutralizing antibodies. In addition, a significant percentage of the general population is not eligible for AAV-mediated gene therapy due to pre-existing immunity. Development of additional viral vectors may overcome this hurdle. Simian virus 40 (SV40)-derived vectors have been reported to transduce different tissues, including the liver, and prevalence of neutralizing a

SUBMITTER: Shi X 

PROVIDER: S-EPMC5948228 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature