Pathway-specific metabolome analysis with ¹⁸O₂-labeled Medicago truncatula via a mass spectrometry-based approach.

ABSTRACT:

Introduction

Oxygen from carbon dioxide, water or molecular oxygen, depending on the responsible enzyme, can lead to a large variety of metabolites through chemical modification.

Objectives

Pathway-specific labeling using isotopic molecular oxygen (¹⁸O₂) makes it possible to determine the origin of oxygen atoms in metabolites and the presence of biosynthetic enzymes (e.g., oxygenases). In this study, we established the basis of ¹⁸O₂-metabolome analysis.

Methods

¹⁸O₂ labeled whole Medicago truncatula seedlings were prepared using ¹⁸O₂-air and an economical sealed-glass bottle system. Metabolites were analyzed using high-accuracy and high-resolution mass spectrometry. Identifi

SUBMITTER: Kera K 

PROVIDER: S-EPMC5948250 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature