Engineering a vitamin B₁₂ high-throughput screening system by riboswitch sensor in Sinorhizobium meliloti.

ABSTRACT:

Background

As a very important coenzyme in the cell metabolism, Vitamin B₁₂ (cobalamin, VB₁₂) has been widely used in food and medicine fields. The complete biosynthesis of VB₁₂ requires approximately 30 genes, but overexpression of these genes did not result in expected increase of VB₁₂ production. High-yield VB₁₂-producing strains are usually obtained by mutagenesis treatments, thus developing an efficient screening approach is urgently needed.

Result

By the help of engineered strains with varied capacities of VB₁₂ production, a riboswitch library was constructed and screened, and the btuB element from Salmonella typhimurium was identified as the best regulatory device. A flow cytometry high-throughput scree

SUBMITTER: Cai Y 

PROVIDER: S-EPMC5948670 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature