High-efficiency derivation of human embryonic stem cell lines using a culture system with minimized trophoblast cell proliferation.

ABSTRACT: BACKGROUND:Due to their extensive self-renewal and multilineage differentiation capacity, human embryonic stem cells (hESCs) have great potential for studying developmental biology, disease modeling, and developing cell replacement therapy. The first hESC line was generated in 1998 by culturing inner cell mass (ICM) cells isolated from human blastocysts using an immunosurgery technique. Since then, many techniques including mechanical ICM isolation, laser dissection, and whole embryo culture have been used to derive hESC lines. However, the hESC derivation efficiency remains low, usually less than 50%, and it requires a large number of human embryos to derive a significant number of hESC lines. Due to a shortage of and restricted access to human embryos, a novel approach with better hESC d

SUBMITTER: Laowtammathron C 

PROVIDER: S-EPMC5948903 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature