Unique and shared inflammatory profiles of human brain endothelia and pericytes.

ABSTRACT:

Background

Pericytes and endothelial cells are critical cellular components of the blood-brain barrier (BBB) and play an important role in neuroinflammation. To date, the majority of inflammation-related studies in endothelia and pericytes have been carried out using immortalised cell lines or non-human-derived cells. Whether these are representative of primary human cells is unclear and systematic comparisons of the inflammatory responses of primary human brain-derived pericytes and endothelia has yet to be performed.

Methods

To study the effects of neuroinflammation at the BBB, primary brain endothelial cells and pericytes were isolated from human biopsy tissue. Culture purity was examined using qPCR and immunocytochemistry. Electrical cell-substrate impedance sensing (ECI

SUBMITTER: Smyth LCD 

PROVIDER: S-EPMC5948925 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature